RNA plays an important role in both the transcription and translation processes. Unlike bacteria, in eukaryotes, RNA will be transported, localized, and locally translated. Therefore, it is meaningful to detect the localization and quantify the RNA. RNA-FISH is one of the most popular methods to quantify and localize RNA in fixed cells. For this reason, we would like to establish the RNA detection in Saccharomyces cerevisiae using fluorescence in situ hybridization (FISH).